Imaging Cell Movements in Egg-Cylinder Stage Mouse Embryos
نویسندگان
چکیده
منابع مشابه
Failure of egg cylinder elongation and mesoderm induction in mouse embryos lacking the tumor suppressor smad2.
smad genes constitute a family of nine members whose products serve as intracellular mediators of transforming growth factor beta signals. SMAD2, which is a tumor suppressor involved in colorectal and lung cancer, has been shown to induce dorsal mesoderm in Xenopus laevis in response to transforming growth factor beta and activins. The smad2 gene is expressed ubiquitously during murine embryoge...
متن کاملDevelopment in vitro of mouse embryos from the two-cell egg stage to the early somite stage.
About 1-3 % of mouse blastocysts, which had initially been cultured from the two-cell stage in chemically defined medium or about 3-5 % of blastocysts which were explanted from the uterus, developed to the early somite stage when cultured in vitro on collagen. Twocell eggs were initially cultivated in chemically defined medium to the blastocyst stage. Blastocysts were then transferred to Eagle'...
متن کاملWhich stage of mouse embryos is more appropriate for vitrification?
Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...
متن کاملEfficacious cryopreservation of 2 - cell stage mouse embryos via
S Introduction ince Whittingham et al. first succeeded in cryopreserving mouse embryos in 1972, there has been remarkable progress in both basic and applied research enabling us to preserve the embryos of at least 13 more mammalian species. Moreover, every process used for freezing and thawing embryos has been refined and extremely simplified. Specifically: 1) The time required for the embryo t...
متن کاملP-30: Developmental Capacity and Blastocyst Formation of Thawed Tetrahedral Versus Non-Tetrahedral 4-cell Stage Mouse Embryos After Vitrification
Background: It was reported in a literature that approximately one third of the 4-cell stage embryos did not exhibit a tetrahedral shape. Non-tetrahedral embryos showed a lower in vitro developmental potential than tetrahedral embryos. Recently vitrification technology has been widely employed for embryo cryopreservation. The objective of this study was to prove our hypothesis that vitrified - ...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2010
ISSN: 1559-6095
DOI: 10.1101/pdb.prot5539